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SPECIES: Tiger moth, Trichoplusia ni
ECACC catalog code: 94020901
Description: Trichoplusia ni ova
Viruses: See Properties
Depositor: Dr M Shaw, Zeneca Pharmaceuticals, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: High density Baculovirus expression of protein.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EX-CELL 401 (Serum free) / EX-CELL 405 (Serum free). Split confluent cultures 1:2 to 1:4 i.e. seeding at 5x10,000 cells/cm2; 5% CO2; 27C. Do not split more than 1:5. Scrape cells to detach from flask.
The Tn5 B1-4 cell line was derived from Trichoplusia ni eggs. They posses a high protein expression potential (up to 28-fold more than SF9 cells ECACC No. 89070101). The cells are attachment-dependent but have also been successfully adapted for growth in suspension.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
- Biotechnol Prog 1993;9:25 - PMID: 7764044
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.