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SPECIES: mouse, BALB/c
TISSUE/ORGAN: testis, Sertoli cell
ECACC catalog code: 88111401
Description: Mouse BALB/c testis sertoli cell
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Androgen, oestrogen and progesterone receptors Products: Transferrin, H-Y Ag, novel retinol-binding protein Applications: Hormone studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Fetal Bovine Serum (FBS). Split confluent cultures 1:20 to 1:200 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C. Cells resuscitated from frozen stock should be split 1:3 at the first passage and thereafter as above.
From primary culture of sertoli cell enriched preparations from normal testes of a 11-13 day old BALB/c nu/+ mice. Cells respond to FSH but not LH; Plasminogen activator secretion is stimulated by FSH and also retinoic acid. Note: only distributed frozen.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1715
- Ann N Y Acad Sci 1982;383:44-68 - PMID: 7046561
- Biol Reprod 1980;23:243-250 - PMID: 6774781
- J Biol Chem 1984;259:3117 - PMID: 6538197
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.