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TUMOR: islet tumor
ECACC catalog code: 94022429
Morphology: Trabecular small cell
Description: Mouse islet cell tumour
Viruses: See Properties
Depositor: Prof OS Pettengill and Dr DS Longnecker, Dartmouth Medical School, Lebanon, NH, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Somatostatin Applications: Study of endocrine pancreas carcinomas, hormone assays.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM:Ham's F12 (1:1) + 2mM Glutamine + 10% Fetal Bovine Serum (FBS) (Heat Inactivated). Split confluent cultures 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
TGP 52 has been isolated from an islet tumour arising in the pancreas of a transgenic Tg (E1a-1-SV40E) Bri18 mouse. These mice carry a pseudogene construct composed of a pancreatic specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 52 cells are SV40T antigen positive and secrete somatostatin, it is one of a series of pancreatic tumour lines deposited at ECACC. Cells transiently produce insulin in culture followed by somatostatin.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 2140
- Carcinogenesis 1994;15:61-65 - PMID: 7904904
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.