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ECACC catalog code: 93022307
Description: Human erythroleukaemia
Viruses: See Properties
Depositor: Mrs M Freshney, The Beatson Institute, Glasgow, UK
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Interleukin 3 (IL-3) and Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) Applications: Tumorigenic studies, analysis of IL-3, GM-CSF and EPO-receptors study of signal transduction of haemopoietic growth factors.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 2-5ng/ml Human Granulocyte Macrophage Colony Stimulating Factor (Human GM-CSF) + 10% Fetal Bovine Serum (FBS). Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37C.
Karyotype: Carries homogeneous chromosomal abnormality (54,X)
TF-1 have been derived from a patient with erythroleukaemia. They show complete growth dependency on GM-CSF or IL-3, and carry a homogeneous chromosomal abnormality (54,X). Constitutive expression of globin genes indicate commitment of TF-1 to the erythroid lineage, although glycophorin A or carbonyl anhydrase I are not expressed. On incubation with hemin or delta-aminolevulinic acid haemoglobin synthesis was induced, whereas TPA induced differentiation into macrophage-like cells. Erythroprotein also sustains short-term growth, does not induce differentiation.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
- Exp Cell Res 1993;208:35-43 - PMID: 8359227
- J Cell Physiol 1989;140:323-334 - PMID: 2663885
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.