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SPECIES: human, Caucasian
ECACC catalog code: 92090213
Description: Human Caucasian glioblastoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Studies on the mechanism for cessation of proliferation in quiescent cells and cells synchronized in G1 phase.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C. Cells may become anchorage-independent if deprived of serum.
Karyotype: Hyperpentaploid, modal No 128-132
Derived from glioblastoma multiform tumour from a 61-year-old Caucasian male. Indefinite lifespan and anchorage independent but can enter viable G1 arrested state when deprived of serum.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1690
- J Cell Physiol 1979;99:43-54 - PMID: 222778
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.