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SPECIES: hamster, Syrian
DSMZ catalog code: ACC 61
Morphology: fibroblastoid adherent monolayer
Susceptibility to viruses: adenovirus 25 * herpes simplex * pseudorabies * reovirus 3 * rubella * vaccinia * vesic. stomatitis (Indiana)
Description: Syrian hamster kidney BHK-21 is a subclone (clone 13) of the parental line established from the kidneys of five unsexed, one-day-old hamsters in 1961; cells can be used for virus replication studies confirmed as hamster with IEF of AST, LDH, MDH, MPI, NP, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: obtained from ATCC (CCL 10), Rockville, Maryland, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% MEM (with Earle's salts) + 10% FBS + 1x non-essential amino acids split confluent culture 1:2 to 1:3 every 2-4 days using trypsin/EDTA; seed out at ca. 0.2 x 105 cells/cm 2 at 37C with 5% CO2 about 20-30 x 106 cells/80 cm 2;
doubling time of ca. 32-50 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (near-diploid with 21% tetraploidy; 40-45,XY; hypetdiploid sideline present at 19%; resembles published description) * isoenzymes (confirmed as hamster with IEF of AST, LDH, MDH, NP, PEP B)
Availability in cell line catalogues: ATCC: CCL 10
- J Natl Cancer Inst 1963;30:795-811
- Virology 1962;16:147-151
- Virology 1965;27:239
- Virology 1965;27:308
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.