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ICLC catalog code: HTL95022
Continuous culture, grown as monolayer
SPECIES: human (72 years old) Karyology: modal chromosome number 56
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: hormone studies; tumorigenicity; receptors for many peptide hormones and neurotransmitters; the cells maintain vectorial electrolyte transport.
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + Ham's F12 (1:1) + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:2-1:3 using trypsin/EDTA; the cells are difficult to disaggregate after tripsinization; seed at 1-3x104 cells/cm2. 37C, 5% CO2. Passage: 60, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with AST, G6PD)
Availability in cell line catalogues: ECACC: 88021101
- Am J Physiol 1984;246:204 - PMID: 6141741
- Proc Natl Acad Sci USA 1980;77:3464 - PMID: 6932031
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.