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ECACC catalog code: 94102702
Description: Human Caucasian aorta, normal smooth muscle
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Smooth muscle cell studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10mM Hepes + 10mM TES + 50µg/ml Ascorbic acid + 10µg/ml Insulin + 10µg/ml Transferrin + 10ng/ml sodium selenite + 30µg/ml Endothelial Cell Growth Supplement + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
The T/G HA-VSMC smooth muscle cell line was derived from the normal aorta of an 11 month old female, blood type O positive. The cell line has been reported to undergo a total of 30 to 35 passages before the onset of senescence.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1999
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.