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SPECIES: human, Caucasian
ECACC catalog code: 89012702
Description: Human colon adenocarcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Carcinoembryonic antigen (CEA) Applications: Tumourigenicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
L-15 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; No CO2; 37C.
Karyotype: 2n = 46
The cells produce a low level of CEA: 0.6ng/1,000,000 cells in 10 days. Cells are tumorigenic in nude mice. Established from a grade IV ulcerating tumour encircling the bowel of an 82 year old Caucasian female. HLA = A32, A33, B27, B35
Research council deposit: No;
Price Code: B;
Validated by: HLA typing (A32,33;B27,35) * isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 231
- Cancer Res 1976;36:4562-4569 - PMID: 1000501
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.