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SPECIES: hamster, golden Syrian
ECACC catalog code: 85011423
Description: Hamster Syrian kidney
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Transformation and virus studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM or GMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
BHK TK- is a derivative of BHK21 (clone 13) rendered TK- by selection in 30µg/ml BUdR. Used widely for research on viruses and on neoplastic transformation especially with polyoma virus.
Research council deposit: Yes;
Price Code: B;
Validated by: isoenzymes (species confirmation)
- Nature 1964;203:1355
- Nature 1966;211:250 - PMID: 4290606
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.