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TUMOR: leukemia, Friend
ECACC catalog code: 91112132
Description: Mouse Friend leukaemic lymphoblast
Viruses: See Properties
Depositor: Dr Y A Wilkinson, Biomedical Sciences Research Centre, University of Ulster, Coleraine, UK
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
SF12 + 2mM Glutamine + 1% AA + 1.05g Sodium Bicarbonate (NaHCO3) + 4ml (1M) NaOH + 10% Horse Serum (HS). Maintain cultures between 3-6x100,000 cells/ml; 5% CO2; 37C.
Karyotype: Chromosome Nos 33-41
Induced by 300ug/ml EMS. Resistant to 2uM ARA-C. Greater than 1.5xCTP level of wild type CLONE 707 approximate 4x level of dCTP of wild type CLONE 707.
Research council deposit: Yes;
Price Code: C;
Validated by: isoenzymes (species confirmation)
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.