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SPECIES: Bombyx mori (silkworm)
DSMZ catalog code: ACC 186
Morphology: large, mostly adherent cells; 90% round cells (singly or aggregates), 10% spindled-shaped cells with long processes
Description: insect - silkworm (Bombyx mori) derived from larvae of a silkworm (species Bombyx mori, family Bombycidae, order Lepidoptera) (Seidenspinner) confirmed as not of primate or murine origin with IEF of AST, PEP B
Depositor: Dr. H. S. Conradt, GBF, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% TC 100 medium + 20% FBS cells are difficult to culture and grow slowly! split confluent culture 1:3 to 1:6 every 3-4 days using a (plastic) cell scraper cells grow without CO2 (keep lid of flask closed) at room temperature (20-25 C) cell harvest of up to 12 x 106 cells/80 cm 2 flask;
doubling time of ca. 50-100 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.