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SPECIES: mouse / mouse
DSMZ catalog code: ACC 146
Morphology: single, round cells in suspension
Transformed by: Mutations Back To Mutations WebPage
Description: mouse myeloma SP2/0-AG14 is a hybrid between BALB/c spleen cells and the myeloma cell line P3X63AG8; cells were described as not synthesizing or secreting immunoglobulin chains, being resistant to azaguanine at 20 ęg/ml, and not growing in HAT medium; cell line is used as a fusion partner for the production of hybridomas secreting specific monoclonal antibodies confirmed as mouse with IEF of LDH, NP, PEP B
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. G. Koehler, Max-Planck-Institute, Freiburg, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
92.5% Iscove's MDM + 7.5% FBS + 2 mM L-glutamine maintain at 0.1-0.5 x 106 cells/ml; split 1:3 to 1:10 every 2-4 days; seed out at ca. 0.5 x 106 cells/ml at 37C with 7.5% CO2 maximal density at 0.5-1.0 x 106 cells/ml;
doubling time of ca. 30 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: isoenzymes (confirmed as mouse with IEF of LDH, NP, PEP B)
Availability in cell line catalogues: ATCC: CRL 1581, ATCC: CRL 1581, ECACC: 85072401
- J Immunol 1981;126:317-321 - PMID: 6935293
- Nature 1978;276:269-270 - PMID: 714156
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.