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TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, T cell
DSMZ catalog code: ACC 53
Morphology: single, round cells in suspension
Description: human T cell leukemia established from the peripheral blood of a 61-year-old man with T cell chronic lymphocytic leukemia (CLL) in 1977 confirmed as human with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Minowada, Fujisaki Cell Center, Okayama, Japan
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.3-1.0 x 106 cells/ml; seed out at ca. 0.5 x 106 cells/ml at 37C with 5% CO2 saturation density at about 1.5 x 10 6 cells/ml;
doubling time of ca. 30-40 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, PCR assays;
Validated by: cytogenetics 1 (near diploid with 4% polyploidy; 46(43-48)<2n>XY, +8, -14, t(3;3)(q11;q27), der(8)t(8;14)(q24;q11)x2, t(8;11)(p21;p12), der(12)t(12;?)(q24;?), der(14)t(8;14)(q24;q11)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD2+ CD3- CD4- CD5+ CD6+ CD7+ CD8- CD13- TCR_/beta-) * isoenzymes (confirmed as human with IEF of MDH, NP) * reverse transcriptase (negative for reverse transcriptase, HTLV-I/II, HIV-1)
- J Immunol 1979;123:1133-1140 - PMID: 313946
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.