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TUMOR: not defined tumor
ECACC catalog code: 94092301
Morphology: Polygonal, tendency to float
Description: Human primitive neuroectodermal tumour
Viruses: See Properties
Depositor: Mrs C Helson, St Agnes Hospital, 305 North St, White Plains, New York, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Vimentin, P53, anti-MIC2 Products: Pro-cholecystokinin (Pro-cck) Applications: Tumour lines should prove useful in understanding the regulation of the cck gene in human neuronal cells and will also provide an `in vitro' system for investigation of the post translational processing of the cck preprohormone.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
SK-PN-DW has been derived from a male patient with a primitive neuroectodermal tumour that expresses cholecystokinin (cck) RNA and synthesises substantial amounts of pro-cck. SK-PN-DW is able to process the pro-hormone, producing immunoreactive cck-like material plus a peptide that immunochemically and chromatographically resembles intact cck c-terminal extension peptide.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 2139
- Cancer Genet Cytogenet 1987;24:75 - PMID: 3024811
- J Clin Endocrinol Metab 1989;69(2):411 - PMID: 2753982
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.