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TISSUE/ORGAN: bone marrow
ECACC catalog code: 94092305
Description: Human neuroblastoma
Viruses: See Properties
Depositor: Mrs C Helson, St Agnes Hospital, 305 North St, White Plains, New York, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: p53, moderate MDR-1 expression Applications: Tumour studies. MRD-1 resistance studies. Extremely sensitive to cis-retinoic acid.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Karyotype: 44XX, monosomic for 10, 11, 13, 14, 19. Both copies of chromosome 2 missing in each cell.
Spontaneously transformed cells from a female patient with neuroblastoma. The tumour was derived from a bone marrow metastasis and is tumourigenic in nude mice. Used in the study of MIBG uptake in the diagnosis and treatment of neuroblastoma. MIBG is a derivative of the antihypertensive agent guanethidine and is used as a radiopharmaceutical. SK-N-DZ is characterised by low saturable kinetics and predominant linear pattern of uptake of MIBG, with a poor ability to maintain high intracellular levels of the drug.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 2149
- Acta Neurophatol 1989;79:217 - PMID: 2480694
- Cancer Res 1993;53:304 - PMID: 8417824
- Int J Oncol 1994;5:1037
- J Natl Cancer Inst 1984;73(1):51 - PMID: 6610792
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.