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SPECIES: human, Caucasian
DSMZ catalog code: ACC 151
Morphology: adherent, epithelial-like cells growing as monolayers
Description: human melanoma derived from tumor tissue (subcutis metastasis) of a 67-year-old Caucasian man with malignant melanoma in 1975 confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Atzpodien & Dr. H. Kirchner, Medical School Hannover, Hannover, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent culture 2-3 times per week using trypsin/EDTA; seed out about 5 x 106 cells/175 cm 2 at 37C with 5% CO2 about 6 x 106 cells/80 cm 2;
doubling time of ca. 30 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x10 6 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (hypertriploid karyotype with 3.5% polyploidy; 80(69-83)<3n>XXY-Y/XXYY, +1, +1, +2, +2, +3, +7, +10, -11, +12, +17, +18, +20, -21, +2mar, inv(6)(p11;q25), der(7)t(7;14)(p22;q22), der(9)t(9;?21)(p11;q11)x2, 14p+, der(15)t(15;?)) * cytogenetics 2 (der(16)t(16;?)(q23;?), der(18)t(18;?)(p11;?), mar: ?der(19)t(1;19)(q12;q13); additonal subclonal rearrangements of ch1: der(1)t(1;?)(p12;?) were present in a few cells) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin- desmin- endothel- GFAP- neurofilament- vimentin+) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
- Proc Natl Acad Sci U S A 1976;73:3278-3282 - PMID: 1067619
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.