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SPECIES: human, Caucasian
TISSUE/ORGAN: bone marrow
ECACC catalog code: 94030304
Description: Human neuroblastoma
Viruses: See Properties
Depositor: Dr PFT Vaughan, Institute for Cardiovascular Research, University of Leeds, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Neurotransmitter studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F12:EMEM (EBSS) (1:1) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Fetal Bovine Serum (FBS). Split confluent cultures 1:10 to 1:100 i.e. seeding at 1x1,000-1x10,000 cells/cm2 every 2-4 weeks; 5% CO2; 37C. Cells reattach slowly and may remain in suspension for several days. NB: do not use after passage 20.
SH-SY5Y is a thrice-cloned sub-line of bone marrow biopsy-derived line SK-N-SH (ECACC No. 86012802). SH-SY-5Y has dopamine-beta-hydroxylase activity and can convert glutamate to the neurotransmitter GABA. Will form tumours in nude mice in approximately 3-4 weeks.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: DSMZ: ACC 209, ICLC: HTL95013
- Cancer Res 1973;33:2643-2652 - PMID: 4748425
- Cancer Res 1978;38:3751-3757 - PMID: 29704
- Cancer Res 1990;50:3422-3428 - PMID: 2334938
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.