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SPECIES: lepidoptera sciaridae diptera - Spodoptera frugiperda (fall armyworm)
TISSUE/ORGAN: pupal ovary
ECACC catalog code: 89070101
Susceptibility to viruses: Ac MNPV * baculovirus
Description: Spodoptera frugiperda pupal ovarian tissue
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: Baculovirus
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
TC100 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS) or SF 900 (Gibco) Serum free. Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 27C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 9% glycerol/91% FBS. Cultures may take up to 10 days to recover normal growth characteristics following cryopreservation and resuscitation.
Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1711
- Biotechnology 1988;6:1406
- Biotechnology 1988;6:47
- In Vitro 1977;13:213-217 - PMID: 68913
- Proc Natl Acad Sci U S A 1985;82:8404-8408 - PMID: 3878519
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.