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SPECIES: rabbit, domestic
ECACC catalog code: 90092702
Morphology: Epithelial. Cells become spindle shaped when confluent
Susceptibility to viruses: herpes simplex * rabbit Shope papilloma virus * reovirus 3 * vaccinia * vesicular stomatitis (Ogden strain)
Description: Rabbit epidermis, cottontail
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus replication studies (may be useful for viruses producing lesions in skin)
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C. Finite lifespan. Maximum of 80 passages expected.
Karyotype: 2n = 42
From minced ear epidermis of adult male cottontail rabbit with papillomatosis. Cells can be cultured continuously at pH 7.4 and 37C but may also be adequately maintained at 25C. Developed for studies of epidermis specific virus replication. Also know as Sf1Ep (NBL-11).
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 68
- Proc Soc Exp Biol Med 1958;98:574
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.