This is the complete description of the cell line that you selected.
By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).
SPECIES: Drosophila, melanogaster
DSMZ catalog code: ACC 130
Morphology: small adherent cells growing in monolayers; a small number of cells is also in suspension
Description: insect - fruitfly (Drosophila melanogaster) established from the late embryo of a Drosophila melanogaster (fruitfly) in 1972; originally the cells were diploid with 5-10% XY, currently the cell line has only XX cells that are now 60-80% tetraploid a mix-up with human or murine cells was excluded with IEF of AST, MDH, PEP B
Depositor: Dr. W. J. Gehring, Biocenter of the University of Basel, Basel, Switzerland
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
85-90% Schneider's Drosophila medium + 10-15% FBS maintain at room temperature without gas exchange (keep lid of flask closed); cells are resistant to trypsination, for propagation use cells in suspension or remove adherent cells with a rubber policeman about twice a week; after thawing cells may have a long lag period (3-7 days) before they start to grow; split confluent culture 1:2 to 1:4 every 2-4 days; seed out at ca. 1-2 x 106 in 80 cm 2 flask at room temperature without CO2 about 15-20 x 106 cells/25 cm 2;
doubling time of ca. 40 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-3 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: isoenzymes (a mix-up with human or murine cells was escluded with IEF of AST and MDH)
- J Embryol Exp Morphol 1972;27:353-365 - PMID: 4625067
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.