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SPECIES: human, Caucasian
DSMZ catalog code: ACC 228
Morphology: epitheloid cells growing adherently in monolayers, often triangle-formed cells
Description: human cervix carcinoma (derivative of HELA) these cells were thought to be 'established from the malignant urinary bladder carcinoma of a 82-year-old Caucasian woman (stage pTa G1-G2) in 1992'; same donor as for cell line SBC-7 (DSM ACC 229); however, DNA fingerprinting and cytogenetic analysis at the DSMZ established unequivocally that this cell line must be considered de facto a subclone of HELA confirmed as human with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Prof. A. J. Ulmer, Forschungsinstitut Borstel, Borstel, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent culture 1:10 to 1:20 every second day using trypsin/EDTA; seed out at about 1 x 106 cells/175 cm 2 at 37C with 5% CO2 cell harvest of about 20-25 x 106 cells/175 cm 2;
doubling time of about 40 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (human near triploid karyotype with 13% polyploidy; 67(64- 70)<3n>XX,-X, +7, -8, -8, -9, +15, -18, -21, +2 mar; der(2)t(2;4)(p24;q12), del(3)(q24.3), add(6)(q25), i(13q), del(17)(p12), add(19)(p13); Ph- reminiscent marker present) * DNA fingerprinting (unique, heterozygous profile with pYNH24 probe) * immunological analysis (cytokeratin+ (100%), desmin-, endothel-, GFAP-, neurofilament+ (5-10%), vimentin+ (100%)) * isoenzymes (confirmed as human with IEF of AST, NP) * reverse transcriptase (reverse transcriptase not detected)
- Immunology 1993;80:151-156 - PMID: 8244458
- J Urol 1993;150:1932-1937 - PMID: 8230540
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.