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DSMZ catalog code: ACC 266
Morphology: epitheloid cells growing adherently as monolayer
Description: human thyroid sarcoma established from the sarcoma of the thyroid gland from a 47-year-old woman in 1985 confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: obtained from the DKFZ Tumorbank, Heidelberg, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent culture 1:2 about once a week using trypsin/EDTA at 37C with 5% CO2 cell harvest of about 0.5-1.0 x 105 cells/cm 2;
doubling time of about 37 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (flat-moded hexaploid/octaploid karyotype with 14% polyploidy; 156(140-188)<6n>XXXX, -X, -X; multiple copies of: i(1p), i(1q), i(3p), i(6p), i(9q), add(5)(p15), add(16)(q23), i(mar); 1-3 dmin/rings in 40% cells; isochromosome) * cytogenetics 2 (formation previously reported in thyroid tumors) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+, desmin-, endothel-, GFAP+, neurofilament-, vimentin+) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase not detected)
- J Cancer Res Clin Oncol (Suppl) 1989;115
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.