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TUMOR: lymphoma, B cell
DSMZ catalog code: ACC 215
Morphology: single round to pleomorph cells growing in suspension
Description: human B cell lymphoma established in 1998 from the pleural effusion of a 35-year-old HIV-positive homosexual man with primary effusion lymphoma and a history of Kaposi's sarcoma; cells are HHV-8-positive, but HIV-negative confirmed as human with IEF of AST, LDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HHV-8+, HIV-, HTLV-I/II-
Depositor: Prof. A. Carbone, Centro di Riferimento Oncologico, Aviano, Italy
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80-85% RPMI 1640 + 15-20% FBS split saturated culture 1:3 every 3-4 days; maintain at ca. 0.4-0.8 x 106 cells/ml; seed out after thawing at ca. 0.5 x 10 6 cells/ml at 37C with 5% CO2 maximal density at ca. 1 x 106 cells/ml;
doubling time of ca. 35-40 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (murine chromosome morphology) * immunological analysis (secretion of a mouse IgG1 monoclonal antibody) * isoenzymes (confirmed as mouse with IEF of AST, NP, PEP B) * reverse transcriptase (reverse transcriptase negative)
- J Muscle Res Cell Motil 1989;10:197-205 - PMID: 2547831
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.