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SPECIES: mouse / mouse
DSMZ catalog code: ACC 156
Morphology: single, round cells in suspension (some cells adhere partly, but trypsin is not necessary to detach these cells)
Description: mouse hybridoma (anti-rat fiber/myosin) this hybridoma cell line was described as secreting a mouse monoclonal antibody (IgG 1 ) that reacts with rat neonatal fibers and, to a lower extent, with all rat adult type 2 fibers in immunofluorescence; it reacts specifically with neonatal MHCs (myosin heavy chain) in Western blotting; established in 1984 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from fetal bovine muscles confirmed as mouse with IEF of AST, MDH, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: Prof. S. Schiaffino, University of Padova, Padova, Italy
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM (4.5 g/L glucose) + 10% horse serum + 2 mM L-glutamine split saturated culture at 1:2 to 1:3 every 2-3 days at 37C with 5% CO2 maximal density at about 1.5-2.0 x 106 cells/ml;
doubling time of ca. 30-40 hours;
frozen with 70% medium, 20% horse serum, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (murine chromosome morphology) * immunological analysis (secretion of a mouse IgG1 monoclonal antibody) * isoenzymes (confirmed as mouse with IEF of AST, MDH, PEP B) * reverse transcriptase (reverse transcriptase not detected)
- Differentiation 1988;38:49-59 - PMID: 3181651
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.