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ECACC catalog code: 86082803
Susceptibility to viruses: poliovirus 3 * vesicular stomatitis
Description: Human choriocarcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Gonadotrophin, lactogen and steroid hormones Applications: Hormone studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F12 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C; medium change or subculture every 36-48 hours necessary.
Karyotype: 2n = 46
The first human, trophoblastic endocrine cell type to be maintained in continuous culture. It was initiated from a malignant gestational choriocarcinoma of the foetal placental. Secretes placental hormones including gonadotrophin, lactogen and steroid hormones. Cellular differentiation of this stem cell type occurs when hormonal precursors are added to the medium. Due to the high glycolytic activity the cells have to be sub-cultured or supplied with fresh media every 36-48 hours.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 98, IZSBS: BS TCL3, DSMZ: ACC 458
- Cancer Res 1968;28:1231 - PMID: 4299001
- In Vitro 1970;6:205 - PMID: 5535575
- J Clin Endocrinol Metab 1972;34:59-61 - PMID: 4332667
- Science 1968;159:1467 - PMID: 5753554
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.