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SPECIES: rat, NEDH
TUMOR: islet tumor
ECACC catalog code: 95071701
Description: Rat islet cell tumour
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Somatostatin, insulin Applications: Study of biology of pancreatic islet cells, synthesis, storage and secretion of insulin and somatostatin
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Established from a transplantable pancreatic tumour of the islets of Langerhans. The tumour originated in an irradiated NEDH inhibited albino rat and was maintained by serial transplantation in Nedh rats. After transplantation into athymic nude mice the cell line RIN-m was established from the fourth nude mouse heterotransplant, 9 days after the tumour was removed cells were exposed to cystine-free medium resulting in the complete elimination of fibroblast-like cells. RIN-m cells secrete insulin and somatostatin. High levels of L-dopa-decarboxylase and membrane bound secretory granules were detected. RIN-5F (95090402) was established as a secondary clone from RIN-m.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 2057
- Diabetes 1982;31:521 - PMID: 6295859
- Proc Natl Acad Sci U S A 1977;74:628 - PMID: 191819
- Proc Natl Acad Sci U S A 1980;77:3519 - PMID: 6106192
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.