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TUMOR: islet tumor
ECACC catalog code: 95090402
Description: Rat islet cell tumour
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Insulin, L-dopa-decarboxylase Applications: Study of pancreatic islet cell biology eg. synthesis, storage and secretion of insulin
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
RIN-5F is a secondary clone of the rat islet tumour cell line RIN-m (ECACC No. 95071701). Cloning was performed by limited dilution in RPMI 1640, 10%FBS and 20% conditioned medium. The conditioned medium contained growth hormones and prolactin being produced by rat pituitary tumour cells GH3 (ECACC No. 87012603). RIN-5F cells secrete insulin, produce high levels of the key APUD enzyme, L-dopa-decarboxylase and contain membrane-bound secretory granules.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 2058
- Diabetes 1982;31:521 - PMID: 6295859
- Endocrinology 1983;112:1070 - PMID: 6129963
- Proc Natl Acad Sci U S A 1977;74:628 - PMID: 191819
- Proc Natl Acad Sci U S A 1980;77:3519 - PMID: 6929511
- Proc Soc Exp Biol Med 1983;175:35
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Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.