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SPECIES: rat, Sprague-Dawley
TISSUE/ORGAN: glomerulal, endothelial cells
DSMZ catalog code: ACC 262
Morphology: adherent epitheloid cells growing as monolayer (after confluency cells tend to grow in multilayers)
Description: rat glomerular endothelial cells established from freshly isolated glomeruli from kidneys of 6-week-old female Sprague-Dawley rats confirmed as rat with IEF of AST, PEP B
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. Holthoefer, University of Helsinki, Helsinki, Finland
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent culture 1:2 to 1:3 twice a week using trypsin/EDTA (cells are only lightly adherent and can be detached by tapping the flask; however, to prepare single cell suspension, trypsin/EDTA treatment is necessary); seed out at ca. 2 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of about 2.5 x 106 cells/cm 2;
doubling time of about 17 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (rat hypertriploid karyotype with 6% polyploidy; 67(67- 72)<3n>; corresponds to published karyotype) * isoenzymes (confirmed as rat with IEF of AST, PEP B) * reverse transcriptase (reverse transcriptase negative)
- Lab Invest 1993;69:183-192 - PMID: 8394476
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.