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DSMZ catalog code: ACC 254
Morphology: adherent, epitheloid cells growing in monolayers
Description: human lung carcinoma established from the supraclavicular tumor cells-containing lymph node of a 71-year-old man with poorly differentiated carcinoma of the lung confirmed as human with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. R. Rizzoli, Hopital Cantonal Universitaire, Geneve, Switzerland
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM (4.5 g/L glucose) + 10% FBS split confluent culture 1:3 every 3-4 days using trypsin/EDTA at 37C with 5-10% CO2 cell harvest of about 20 x 10 6 cells/80 cm 2;
doubling time of about 40-50 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (confirmed as human karyotype) * DNA fingerprinting (unique DNA proifle with multilocus (gtg)5 probe) * immunological analysis (cytokeratin+, desmin-, endothel-, GFAP-, neurofilament-, vimentin-) * isoenzymes (confirmed as human with IEF of AST, NP) * reverse transcriptase (reverse transcriptase not detected)
- Br J Cancer 1975;32:373-379 - PMID: 1233082
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.