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SPECIES: human, Caucasian
TUMOR: sarcoma, Ewing's
DSMZ catalog code: ACC 260
Morphology: adherent cells growing in monolayers, sometimes growing loosely adherent in clusters/clumps
Description: human Ewing's sarcoma established from the primary tumor of a 19-year-old Caucasian man with Ewing's sarcoma of the humerus in 1984 confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
85% RPMI 1640 + 15% FBS split confluent culture 1:4 every 5-6 days using trypsin/EDTA (occasional loosely adherent clumps can be detached by tapping the flask); seed out at ca. 2 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of about 10 x 106 cells/80 cm 2;
doubling time of about 60 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2-4 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (flat-moded, near-triploid karyotype; 69(63- 72)<3n>XXY/XXYY,+1,-4,-8,+9,+12,+15,-17,+18,-22, der(9)t(9;11)(p13;q11)t(11;22)(q24;q12)x2, add(14)(p11); carries two masked copies of 11;22 translocation associated with sarcoma of Ewing) * DNA fingerprinting (unique DNA profile with multilocus probe (gtg)5) * immunological analysis (cytokeratin-, desmin-, endothel-, GFAP-, neurofilament-, vimentin+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (reverse transcriptase not detected)
Availability in cell line catalogues: ATCC: HTB 166
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.