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SPECIES: rabbit, New Zealand white
ECACC catalog code: 95081046
TRANSFORMED BY: SV40
Description: Rabbit corneal epithelial cells, SV40 transformed
Viruses: See Properties
Depositor: Dr K Araki-Sasaki, Department of Ophthalmology, Osaka University Medical School, Osaka, JAPAN (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Corneal epithelial research
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM:Ham's F12 (1:1) + 2mM Glutamine + 5µg/ml Insulin + 10ng/ml EGF + 0.1µg/ml Choleratoxin + 0.5% Dimethyl Sulphoxide (DMSO) + 15% Fetal Bovine Serum (FBS) (Heat Inactivated). Split confluent cultures 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
The RCE cell line, also known as K'RCE, was established after infection of primary cultured rabbit (New Zealand albino) corneal epithelial cells with a recombinant SV40 vector. The cells exhibit a cobblestone-like appearance and stratification, including desmosome formation and microvilli development. Expression of keratin AE1 was always detectable whereas AE5 was expressed only at confluency.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Invest Ophthalmol Vis Sci 1993;34:2665
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Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.