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ECACC catalog code: 94020810
Description: Rat pituitary adenoma
Viruses: See Properties
Depositor: Dr M Jutisz, Univ Pierre and Marie Curie, CNRS 1449, 75252 Paris, FRANCE
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Gonadotrophin-releasing hormone receptors Products: Gonadatrophs Applications: Study of gonadotrophic functions of pituitary gland and regulation of pituitary gonadotrophin gene expression.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM:EMEM (EBSS) (1:1) + 2mM Glutamine + 0.2mg/ml BSA + 0.5% Non Essential Amino Acids (NEAA) + 5% Nu-Serum (Collab)+ 2ng/ml EGF. Split confluent cultures 1:6 i.e. seeding at 2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 10% CO2; 34C. Saturation density is reached at 1x 100,000 cells/cm2.
RC-4B/C1 is a clone of the parent RC-4B/C which was established from a 3 year-old CAI/FMaixF344/FMai(F1) male rat pituitary adenoma. Staining studies have shown the cell line contains many bi-hormonal cells with FHSß, LHß and prolactin. Electron microscopy shows the appearance of endogenous C-type rat retroviral particles.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- In Vitro 1990;26:431
- Neuroendocrinology 1991;54:267 - PMID: 1944812
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IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.