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SPECIES: rat, BDIX
TISSUE/ORGAN: brain, fetal
ECACC catalog code: 93040121
Description: Rat, BDIX, fetal brain
Viruses: See Properties
Depositor: Dr J Roscoe, Dept. of Histopathology, University College and Middlesex School of Medicine, London, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Study of carcinogenesis
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 15-20% Fetal Bovine Serum (FBS). Split confluent cultures 1:5 to 1:10 i.e. seeding at 1-2x100,000 cells/cm2 using 0.25% trypsin/EDTA; 10-15% CO2; 37C. Cultures should reach 2x1,000,000 cells per 25cm² flask. Subculture weekly.
The BE11 culture was derived 2 days after a single injection of buffer into pregnant rats. The cells do not form colonies in agar and are not tumourigenic in BDIX rats. Plasminogen activator activity is barely detectable. They are suitable controls for BE10 (Early) cells (93040118).
Research council deposit: Yes;
Price Code: C;
- Br J Cancer 1980;42:756 - PMID: 7193044
- Carcinogenesis 1986;7:477 - PMID: 3081277
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.