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SPECIES: rat, BDIX
TISSUE/ORGAN: fetal brain
TUMOR: brain malignant
ECACC catalog code: 93040120
Description: Rat, BDIX, fetal brain, malignant
Viruses: See Properties
Depositor: Dr J Roscoe, Dept. of Histopathology, University College and Middlesex School of Medicine, London, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Plasminogen activator activity Applications: Study of carcinogenesis, tumourigenicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 15% Fetal Bovine Serum (FBS). Split confluent cultures 1:10 to 1:20 i.e. seeding at 1-2x100,000 cells/25cm² using 0.25% trypsin/EDTA; 10-15% CO2; 37C.
Malignant cells produced on passaging BE10-Intermediate (93040119) beyond passage 40. The cells possess tissue plasminogen activator activity, are tumourigenic in BDIX rats and form colonies in agar.
Research council deposit: Yes;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- J Natl Cancer Inst 1978;61:381
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.