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SPECIES: rat, BDIX
ECACC catalog code: 93040125
Description: Rat, BDIX, brain, pre-malignant
Viruses: See Properties
Depositor: Dr J Roscoe, Dept. of Histopathology, University College and Middlesex School of Medicine, London, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Study of carcinogenesis
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 15% Fetal Bovine Serum (FBS). Split confluent cultures 1:10 to 1:20 i.e. seeding at 1-2x100,000 cells/25cm² using 0.25% trypsin/EDTA; 10-15% CO2; 37C.
Clone of the line BE10-Intermediate (93040119). The cells have elevated levels of tissue plasminogen activity but do not grow in agar or in BDIX rats. The cells should not be used beyond passage 24 as they start to form tumours.
Research council deposit: Yes;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Br J Cancer 1980;41:992 - PMID: 7426322
- Br J Cancer 1980;42:756 - PMID: 7193044
- Br J Cancer 1982;46:965 - PMID: 7150409
- Br Med Bull 1980;1:33 - PMID: 7020827
- Nature 1976;262:314 - PMID: 958380
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.