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SPECIES: fish - trout, rainbow
DSMZ catalog code: ACC 56
Morphology: adherent, fibroblastoid cells growing as monolayers
Description: fish rainbow trout liver established from the liver of a rainbow trout
Depositor: Dr. M. Kohlpoth, Akademie fuer Tierschutz, Neubiberg, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Medium 199 (25 mM HEPES) with Earle's salts with L-glutamine + 10% FBS cell growth is extremely slow; split confluent culture 1:2 every 7 days using TNE (50 mM Tris, 0.1 M NaCl, 5 mM EDTA, pH 7.5) at +4C for 10 min; culture at room temperature (about 20C) without CO2 (keep lid of flask closed); cells can be stored temporarily (up to 1 week) at 4C; seed out at ca. 0.1 x 106 cells/ml at room temperature (about 20C) without CO2 about 5 x 106 cells/175 cm 2 - saturation density of ca. 10 x 106 cells/175 cm 2;
doubling time of about 160 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 106 cells/ampoule;
contamination was eliminated by the depositor with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization assays;
- Zbl Bakt Hyg B 1985;180:480-504
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.