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SPECIES: quail, Japanese
ECACC catalog code: 93120831
Morphology: Spindle shaped and round
Susceptibility to viruses: avian encephalomyelitis * * rabies * Rous sarcoma virus * sub group A, avian sarcoma
Description: Quail Japanese fibrosarcoma
Viruses: See Properties
Depositor: Dr M Ferrari, Instituto Zooprofilattico, Brescia, ITALY
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F10 + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C; subculture at about 75% confluence; cells can be dislodged by tapping flask against the palm of hand; use of 0.5% polyvinylpyrrolidine (PVP) in trypsin solution can increase viability; if trypsin is used neutralize and centrifuge to remove additive; cell growth is very slow.
Derived from methylcholanthrene-induced fibrosarcoma of Japanese quail. The cells are susceptible to several viruses including sub group A avian sarcoma, avian encephalomyelitis, Marek's disease herpes virus and rabies virus.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1708, IZSBS: BS TCL 52
- Cell 1977;11:95 - PMID: 194709
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.