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SPECIES: mouse, C57BL/6
TISSUE/ORGAN: fibroblast, embryonic
DSMZ catalog code: ACC 210
Morphology: adherent fibroblast-like cells growing as monolayer
Description: mouse embryo/fetus calvaria fibroblasts established from the calvaria of an embryo/fetus C57BL/6 mouse; described to differentiate to osteoblasts and to produce collagen confirmed as mouse with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative
Depositor: obtained from the RIKEN Cell Bank (RCB1126), Tsubuka Science City, Ibaraki, Japan
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80-90% alpha-MEM + 10-20% FBS + 2 mM L-glutamine split confluent culture 1:3 to 1:4 once or twice a week using trypsin/EDTA; seed out at about 1-2 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of ca. 6-7 x 106 cells/80 cm 2 or 9 x 106 cells/175 cm 2;
doubling time of ca. 2-3 days;
frozen with 70% medium, 20% FBS, 10% DMSO at ca. 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (human hyperdiploid karyotype with multiple rearrangements; 68-74<3n>X,-Y/der(Y), +1, +6, +7, -10, -13, -17, +4mar; der(1)t(1;?)(p33;?), der(1)t(1;?)(p33;?), der(6)t(6;?)(q15;?), der(19)t(19;?)(p13;?); 19p13 breakpoint is associated wit) * cytogenetics 2 (gastric carcinoma) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin-) * reverse transcriptase (reverse transcriptase negative)
- Virchows Arch B Cell Pathol 1993;63:335-343
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.