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SPECIES: marsupial - potoroo
ECACC catalog code: 91013163
Susceptibility to viruses: vesic. stomatitis (Indiana)
Description: Potoroo kidney
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Chromosome studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:3 i.e. seeding at 4-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: Modal No 11
Derived from kidney of normal adult female Potorous tridactylis in 1962, believed to be the first permanent cell line of marsupial origin to be established. Important because of low number, large size and distinct morphology of chromosomes of this mammalian species.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 35
- Cytogenetics 1964;3:19
- Nature 1962;194:406
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.