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SPECIES: mouse, NIH 3T3
ECACC catalog code: 94090902
TRANSFORMED BY: Moloney-MuLV
Description: Mouse NIH 3T3 transformed with Moloney murine leukaemia mutants
Viruses: See Properties
Depositor: Dr J Liechty / Dr S K Spratt, Somatix Therapy Corporation, Alameda, California, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Ecotropic helper-free recombinant retroviral packaging cell line. Valuable for `in vivo' gene transfer studies aimed at cell lineage analysis and development of human gene replacement therapy.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS) (NB. The results obtained by the depositor were gained using 10% Calf Serum (CS)). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5-10% C02, 37C.
From a set of packaging cell lines useful for generating helper-free recombinant retroviruses with ectotropic host ranges. Two mutant Moloney murine leukaemia virus-derived proviral genomes carrying complementary mutations in the gag-pol or env regions were sequentially introduced into NIH 3T3 cells by co-transformation. Each genome contained a deletion of the Psi sequence necessary for the efficient encapsidation of retroviral genomes into virus particles and additional alterations at the 3'end of the provirus. Psi-CRE can be used to isolate clones that stably produce high titres (1000,000 cfu/ml) of recombinant retroviruses with ectotropic host ranges, viral producers derived from Psi-CRE do not transfer the packaging function or yield helper virus.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Proc Natl Acad Sci U S A 1988;85:6460 - PMID: 3413107
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.