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SPECIES: mouse, NIH Swiss
DSMZ catalog code: ACC 238
Morphology: adherent fibroblastoid cells growing as monolayers
Description: Swiss mouse embryo - NIH-3T3 derivative (retroviral packaging line) subclone of the Swiss mouse embryo cell line NIH-3T3; PSI-2 cells are infected with a replication deficient MLV virus; cells can be used as 'packaging cell line'; classified as risk category S1 according to the German Central Commission for Biological Safety (ZKBS) confirmed as mouse with IEF of AST, NP
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. W. Dirks, DSMZ, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 10% FBS split confluent culture 1:10 to 1:20 every 2-3 days using trypsin/EDTA at 37C with 5% CO2 cell harvest of about 2-4 x 105 cells/cm 2;
doubling time of about 34 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (murine hypotetraploid karyotype; centric fragment present at 74%; ring present at 48%; 68/69(64-74)<4n>; a submetacentric marker arising by centric fusion present at 52%) * isoenzymes (confirmed as mouse with IEF of AST, NP) * reverse transcriptase (reverse transcriptase not detected)
- Cell 1983;33:153-159 - PMID: 6678608
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.