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TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, acute myeloid
DSMZ catalog code: ACC 139
Morphology: single, round cells in suspension
Description: human acute myeloid leukemia (derivative of HL-60) allegedly 'established from the peripheral blood of a 38-year-old woman with acute myeloid leukemia (AML FAB M4) in relapse in 1985'; DNA fingerprinting showed unequivocally that PLB-985 is a subclone of cell line HL-60 confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. T. A. Rado, University of Alabama at Birmingham, Alabama, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at ca. 0.5 x 106 cells/ml; minimum density at 0.2 x 106 cells/ml; seed out at ca. 1 x 106 cells/ml at 37C with 5% CO2 maximal density at about 1.5 x 106 cells/ml;
doubling time of ca. 24 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near diploid with 4% polyploidy; 46(44-50)<2n>X, -X, +6, dm, dup(1)(q21q41), del(3)(p21), del(6)(q21)x2) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13+ CD14- CD15+ CD19- CD33+) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
- Blood 1987;70:372-378 - PMID: 3475136
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.