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ECACC catalog code: 85061101
Description: Rat liver hepatoma
Viruses: See Properties
Depositor: Dr S J Goss, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Resistance and differentiation studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Probably expresses other traits typical of H4-11-E derivatives. It is a derivative of an Fu5 derivative. Hat sensitive, HPRT-, resists 6ug/ml thioguanine. Highly differentiated.
Research council deposit: Yes;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Biochimie 1974;56:1603-1611 - PMID: 4157008
- J Cell Sci 1984;68:285 - PMID: 6149229
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.