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TISSUE/ORGAN: fibroblast, embryonic
ECACC catalog code: 95110215
TRANSFORMED BY: gibbon ape Leukaemia Virus
Description: Mouse embryo fibroblast, GALV-based retrovirus
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus packaging cell system
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
PG13 cells were derived from TK-NIH/3T3 cells. This retrovirus packaging cell line is based on the gibbon ape Leukaemia Virus (GaLV). Introduction of retroviral vectors results in the production of retrovirus virions capable of infecting cells from many species excluding mice. Introduced DNA's may be lost after long-term passage. Selection against loss can be performed by growing the cells in medium with dialysed FBS and100nM amethopterin for 5 days followed by cultivation in HAT and untreated FBS for 5 days. Maintain cells for 4 days in HT medium to avoid toxic effects of residual amethopterin. Virus production has not been observed, but there is the risk that cells produce a virus similar to GaLV.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 10686
- Biotechniques 1989;7:980 - PMID: 2631796
- J Virol 1991;65:2220 - PMID: 1850008
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.