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TUMOR: leukemia, T cell
DSMZ catalog code: ACC 38
Morphology: single, round to oval cells in suspension
Description: human T cell leukemia established from the pleural effusion of a 6-year-old girl with acute lymphoblastic leukemia (ALL) in second relapse confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. L. Pegoraro, University of Turin, Turin, Italy
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-2.0 x 106 cells/ml at 37C with 5% CO2 maximal density of about 3-4 x 106 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near-diploid karyotype with 9% near-tetraploid and 15% polyploid sidelines; 45/46(43-47)<2n>X,-X/XX, -10, +14; sdl with +add(?15)(p11), add(1)(p32)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD2+ CD3- CD4- CD5+ CD6+ CD7+ CD8+ CD13- CD19-) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (negative for reverse transcriptase, HTLV-I/II, HIV-1)
Availability in cell line catalogues: ICLC: HTL00006
- J Natl Cancer Inst 1985;75:285-290 - PMID: 2410652
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.