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SPECIES: mouse / mouse
DSMZ catalog code: ACC 166
Morphology: single cells in suspension
TRANSFORMED BY: SV40
Description: mouse hybridoma (anti-p53) established by fusion of NS-1 myeloma cells and spleen cells from a male BALB/c mouse immunized with SV40-transformed, 3T3 clone 4 mouse cells; cells were reported to secrete an IgG 2b antibody recognizing p53 protein present in nuclei of SV40-transformed cells confirmed as mouse with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% Dulbecco's MEM + 20% FBS maintain at 0.5-5 x 105 cells/ml; split 1:5 every 2-3 days at 37C with 5% CO2 maximal density at 1-2 x 106 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: TIB 116
- J Virol 1980;34:752-763 - PMID: 6155477
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.