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SPECIES: mouse / mouse
DSMZ catalog code: ACC 188
Morphology: single cells growing in suspension
Description: mouse hybridoma (anti-SV40 large T viral antigen) established by fusion of NS-1 myeloma cells and spleen cells from a female BALB/c mouse immunized with SVT-2, an SV40-transformed BALB/c mouse cell line; cells reportedly secrete an IgG 1 antibody recognizing denaturation-sensitive antigenic determinants unique to large T antigen confirmed as mouse with IEF of AST, NP, PEP B
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% Dulbecco's MEM + 20% FBS + non-essential amino acids optimal split ratio 1:2 every 2-3 days; maximum density of about 1.0 x 106 cells/ml should not be exceeded at 37C with 5% CO2 cell harvest of about 0.5-1.0 x 106 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (140(134-155); predominantly mouse chromosomal morphology with 3 iso-chromosome markers present in most cells) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: TIB 115
- J Virol 1980;34:752-763 - PMID: 6155477
- J Virol 1981;37:478-482 - PMID: 6163870
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.