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DSMZ catalog code: ACC 162
Morphology: adherent cells growing in monolayers
Description: human pancreas adenocarcinoma established in 1985 from the liver metastasis of a primary pancreatic adenocarcinoma from a 64-year-old woman; sister cell line of PA-TU-8988S (DSM ACC 204) confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. P. Elsaesser, University of Marburg, Marburg, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 5% FBS + 5% horse serum + 2 mM L-glutamine split confluent culture 1:3 to 1:5 every 3-5 days using trypsin/EDTA at 37C with 5% CO2 cell harvest of about 20-30 x 106 cells/75 cm 2;
doubling time of about 22-30 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (human near-triploid karyotype with 2% polyploidy; 69(63- 70)<3n>XXX, +3, -4, +6, +6, +8, +11, -15, -16, -17, -19, +20, -21, -22, +mar, der(1)t(1;?)(p11;?), der(3) t(3;?)(q11;?), i(6p), del(6)(q23)x2, der(8)t(8;?)(q24.3;?), der(8) (8;?)) * cytogenetics 2 ((p11;?)x2, der(11)t(11;?)(p13;?)x2, del(12)(p12.3), der(?)t(14;?)(q11;?), del(17)(p12), der(17) t(17;?)(p13;?); 1p11 and 8q24 breakpoints previously reported in pancreatic cancer) * DNA fingerprinting (unique profile with (gtg)5 and pYNH24 probes, but identical with PA-TU-8988S) * immunological analysis (cytokeratin + (100%), desmin -, endothel -, GFAP -, neurofilament -, vimentin + (100%)) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase not detected)
- Virchows Arch B Cell Pathol 1992;61:295-306
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.