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ECACC catalog code: 85011417
Description: Mouse myeloma
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Fusion partner, hybridization and tumourigenicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C. Rapid cell death occurs when cell concentration exceeds 1,000,000 cells/ml.
Derivative of P3X63Ag8, known as the Kearney line, the cell line is a non secretor. HGPRT- and HAT sensitive. It is useful for fusion with spleen cells to produce hybridomas or for hybridization studies with other myelomas or lymphomas.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species ocnfirmation)
Availability in cell line catalogues: ATCC: CRL 1597
- Curr Top Microbiol Immunol 1978;81:1-7 - PMID: 567551
- J Exp Med 1979;150:580 - PMID: 90108
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.